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Biosesang Inc 5x sds loading buffer
5x Sds Loading Buffer, supplied by Biosesang Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+sds+loading+buffer/buffer+ripa/pm41828723-231-11-15
Average 86 stars, based on 1 article reviews
5x sds loading buffer - by Bioz Stars, 2026-09
86/100 stars

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Nucleic Acid Electrophoresis:

Article Title: Neuroprotective Effect of the Combined Extract of Mentha piperita and Cornus officinalis Against Neuronal Cell Death and Scopolamine-Induced Memory Impairment.
Article Snippet: .. Protein samples were boiled for 10 min at 100 ◦C in 5X SDS loading buffer (BIOSESANG), separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and then transferred onto 0.45-μm polyvinylidene fluoride (PVDF) membranes (Merck Millipore, Burlington, MA, USA). .. The membranes were blocked with 5% bovine serum albumin (Bovogen, Melbourne, Australia) for 1 h at room temperature and incubated overnight at 4 ◦C with primary antibodies against BDNF (ab108319; Abcam, Cambridge, UK), total (t)-AKT (#9272; Cell Signaling Technology, Danvers, MA, USA), phosphorylated (p)-AKT (#9275; Cell Signaling Technology), t-ERK (#9102; Cell Signaling Technology), p-ERK (#9101; Cell Signaling Technology), t-CREB (#9197; Cell Signaling Technology), p-CREB (#9198; Cell Signaling Technology), and β-actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA).

Article Title: Neuroprotective Effect of the Combined Extract of Mentha piperita and Cornus officinalis Against Neuronal Cell Death and Scopolamine-Induced Memory Impairment
Article Snippet: .. Protein samples were boiled for 10 min at 100 °C in 5X SDS loading buffer (BIOSESANG), separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and then transferred onto 0.45-μm polyvinylidene fluoride (PVDF) membranes (Merck Millipore, Burlington, MA, USA). .. The membranes were blocked with 5% bovine serum albumin (Bovogen, Melbourne, Australia) for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies against BDNF (ab108319; Abcam, Cambridge, UK), total (t)-AKT (#9272; Cell Signaling Technology, Danvers, MA, USA), phosphorylated (p)-AKT (#9275; Cell Signaling Technology), t-ERK (#9102; Cell Signaling Technology), p-ERK (#9101; Cell Signaling Technology), t-CREB (#9197; Cell Signaling Technology), p-CREB (#9198; Cell Signaling Technology), and β-actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA).

Article Title: Coactosin-like F-actin binding protein (Cotl1) plays a key role in adipocyte differentiation and obesity
Article Snippet: Total protein was lysed with radioimmunoprecipitation (RIPA) buffer (BIOSESANG, Seongnam, Korea) containing phenylmethylsulfonyl fluoride (PMSF; Abcam, Cambridge, UK) and phosphatase inhibitor cocktail (PI; Sigma–Aldrich, St. Louis, MO, USA). .. Protein samples were boiled for 10 min at 100 °C after adding 5X SDS loading buffer (BIOSESANG, Seongnam, Korea) and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.45-μm polyvinylidene fluoride (PVDF) membranes (Millipore, Burlington, MA, USA). .. The membranes were blocked with 5% bovine serum albumin (Bovogen, Melbourne, Australia) for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies.

Article Title: Coactosin-like F-actin binding protein (Cotl1) plays a key role in adipocyte differentiation and obesity.
Article Snippet: Total protein was lysed with radioimmunoprecipitation (RIPA) buffer (BIOSESANG, Seongnam, Korea) containing phenylmethylsulfonyl fluoride (PMSF; Abcam, Cambridge, UK) and phosphatase inhibitor cocktail (PI; Sigma–Aldrich, St. Louis, MO, USA). .. Protein samples were boiled for 10min at 100 °C after adding 5X SDS loading buffer (BIOSESANG, Seongnam, Korea) and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.45-μm polyvinylidene fluoride (PVDF)membranes (Millipore,Burlington,MA,USA).Themembraneswere blocked with 5% bovine serum albumin (Bovogen, Melbourne, Australia) for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies. .. Then the membranes were incubated with the appropriate HRPconjugated secondary antibodies and developed with ECL advance reagent (GenDEPOT, Katy, TX, USA).

other:

Article Title: FLEX: genetically encodable enzymatic fluorescence signal amplification using engineered peroxidase.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-V5 Tag Monoclonal Antibody (mouse) Invitrogen Cat # R960-25; RRID: AB_2556564 Alexa Fluor 647 IgG mouse Invitrogen Cat # A28181; RRID: AB_2536165 Streptavidin-HRP Thermo Fisher Scientific Cat # 21126 Streptavidin, Alexa Fluor 647 conjugate Invitrogen Cat # S21374 Recombinant DNA V5-APEX2-NES Mishra et al.16 N/A Sec61B-V5-APEX2 Lee et al.11 N/A APEX2-V5-PLIN2 Mishra et al.16 N/A V5-APEX2-Sec61B Lee et al.11 N/A V5-APEX2-NLS Lee et al.11 N/A TMEM192-V5-APEX2 This paper N/A Mito-mEosEM Fu et al.10 Addgene #132706 Mito-BFP Lee et al.11 Addgene # 49151 CD63-V5-APEX2 This paper N/A TOM20-V5-APEX2 Lee et al.11 N/A Matrix-V5-APEX2 Lee et al.11 N/A Chemicals, Peptides and Recombinant Proteins desthiobiotin-Phenol Mishra et al.16 N/A Azido-PEG3-biotin Alfa aesar Cat #J64996.MB THPTA Sigma Aldrich Cat # 762342 Bafilomycin A1 Sigma Aldrich Cat #B1793 U18666A Medchemexpress fisher scientific Cat # 50-187-2169 PHEMS buffer Montanaro et al.43 N/A Amplex Red Sigma Aldrich Cat # 90101 LysoTracker red Invitrogen Cat # L7528 Horseradish Peroxidase Sigma Aldrich Cat # P8375 H2O2 Sigma Aldrich Cat # H1009 LipiDye Funakoshi Ca t# FNK-FDV-0010 DMSO-D6 Cambridge Isotope Laboratories Cat # DLM-10-100 DAPI Merck (Millipore) 28718-90-3 Dulbecco‘s phosphate-buffered saline (DPBS) Gibco Cat # 21300025 RIPA lysis buffer ELPISBIO Cat # EBA-1149 Protease inhibitor cocktail Invitrogen Cat # 78438 PEI (polyetherimide) Polyscience Cat # 23966-1; CAS: 9002-98-6 Lipofectamine 2000 Invitrogen Cat #11668019 4% paraformaldehyde (PFA) solution Chembio Cat # CBPF-9004 5X SDS loading buffer Biosesang Cat # SF2002-110-00 Glutaraldehyde Electron Microscopy Sciences Cat #16200 Osmium tetroxide Electron Microscopy Sciences Cat #19150 Uranyl acetate Electron Microscopy Sciences Cat #22400 EMBed-812 embedding kit Electron Microscopy Sciences Cat #14120 Uranyless Electron Microscopy Sciences Cat #22409 Lead citrate Electron Microscopy Sciences Cat#22410 Potassium Ferrocyanide Electron Microscopy Sciences SKU#26604-01 (Continued on next page) e1 Cell Chemical Biology 31, 502–513.e1–e6, March 21, 2024

Magnetic Beads:

Article Title: Identification of proteomic landscape of drug-binding proteins in live cells by proximity-dependent target ID.
Article Snippet: Resource Identification of proteomic landscape of drug- binding proteins in live cells by proximity-dependent target ID

Recombinant:

Article Title: Identification of proteomic landscape of drug-binding proteins in live cells by proximity-dependent target ID.
Article Snippet: Resource Identification of proteomic landscape of drug- binding proteins in live cells by proximity-dependent target ID

Lysis:

Article Title: Identification of proteomic landscape of drug-binding proteins in live cells by proximity-dependent target ID.
Article Snippet: Resource Identification of proteomic landscape of drug- binding proteins in live cells by proximity-dependent target ID

Protease Inhibitor:

Article Title: Identification of proteomic landscape of drug-binding proteins in live cells by proximity-dependent target ID.
Article Snippet: Resource Identification of proteomic landscape of drug- binding proteins in live cells by proximity-dependent target ID

Bicinchoninic Acid Protein Assay:

Article Title: Identification of proteomic landscape of drug-binding proteins in live cells by proximity-dependent target ID.
Article Snippet: Resource Identification of proteomic landscape of drug- binding proteins in live cells by proximity-dependent target ID

Incubation:

Article Title: Coactosin-like F-actin binding protein (Cotl1) plays a key role in adipocyte differentiation and obesity.
Article Snippet: Total protein was lysed with radioimmunoprecipitation (RIPA) buffer (BIOSESANG, Seongnam, Korea) containing phenylmethylsulfonyl fluoride (PMSF; Abcam, Cambridge, UK) and phosphatase inhibitor cocktail (PI; Sigma–Aldrich, St. Louis, MO, USA). .. Protein samples were boiled for 10min at 100 °C after adding 5X SDS loading buffer (BIOSESANG, Seongnam, Korea) and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.45-μm polyvinylidene fluoride (PVDF)membranes (Millipore,Burlington,MA,USA).Themembraneswere blocked with 5% bovine serum albumin (Bovogen, Melbourne, Australia) for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies. .. Then the membranes were incubated with the appropriate HRPconjugated secondary antibodies and developed with ECL advance reagent (GenDEPOT, Katy, TX, USA).



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